Cross-axis synchronous flow-through coil planet centrifuge for large-scale preparative counter-current chromatography : II. Studies on partition efficiency in short coils and preparative separations with multilayer coils

Abstract

A series of experiments was conducted to evaluate the partition efficiency of the cross-axis synchronous flow-through coil planet centrifuge. The preliminary studies indicated that short coils (5 m) mounted on 15- and 25-cm diameter holders in either a central or a lateral location produce a high partition efficiency of over 100 theoretical plates. Under the optimum operating conditions, the preparative capability of the countercurrent chromatograph was demonstrated on gram-scale separations of DNP-amino acids and dipeptides in a pair of multilayer coils connected in series with a total capacity of 1600 ml.