Topraktan İzole Edilen Bacillus sp. Suşlarının Lipid Üretim Kapasitelerinin Taranması ve Farklı Boyama Teknikleri ile Lipid Boyama

Abstract

Microbial lipids have been attracting more and more attention in recent years as promising raw 
 materials for the production of biodiesel and value-added compounds. In the current economic and 
 environmental situation, finding new renewable sources of lipids will be crucial. Recent research has 
 necessitated the search for new efficient microbial producers with lipid production efficiency. For this 
 purpose,50 Bacillus sp. strains previously isolated from the soil were screened for their lipid production 
 capacity. As a result of the production using a single-cell oil production medium, only two Bacillus spp. 
 strains showed growth. These bacteria were named as Bacillus sp. E40 and Bacillus sp. E226. Bacteria 
 were then characterized in terms of their biomass, lipid yields, and lipid contents. The biomass of 
 Bacillus sp. E40 and E226 bacterial isolates, were 0,28 and 0,22 g.L-1, respectively. The highest lipid 
 content was reached by E226 with 54.5%. Bacillus sp. E40 lipid content was determined as 46.4%. In 
 addition, both intracellular lipid staining and colony staining demonstrated the presence of lipids. With 
 this study, it was reported for the first time that local Bacillus sp. strains isolated from soil have lipid 
 producing capacity.