Tolerance induction with aggregated bovine gamma globulin in C57B1-6 mice.

Abstract

SummaryBovine γ-globulin aggregated by treatment with bis-diazotized benzidine was fractionated by Na2SO4 precipitation and elution from a Bio-Gel A column to give a highly aggregated though soluble fraction with a molecular weight of about 107. This material administered to C57B1/6 mice still produced tolerance in doses above 0.2 mg comparable to that produced by aggregate-free BGG. By contrast, Balb/C mice require totally monomeric BGG for tolerance induction. The difference in behavior to BGG of these strains is reflected by the differences in ability of their reticuloendothelial systems to filter and clear BGG.