To Study the Effect of Oxygen Carrying Capacity on Exosomes of Red Blood Cells in Different Storage Times

Abstract

Background: Blood is a fluid tissue composed of blood cells and plasma. It mainly transports oxygen, nutrients and metabolites for various tissues and organs of the body. Among them, red blood cells are the important means of oxygen transport. After the blood is stored for a period of time, the structure and properties of the red blood cells will change, which results in a decrease in the oxygen carrying capacity, and the change in the oxygen carrying capacity will also have a certain impact on their exosomes. Methods: Therefore, our research is to detect some carry oxygen function related indicators of red blood cells in different storage times, including effective oxygen uptake (Q), P50, 2, 3-DPG, Na+-K+-ATP. Electron microscopy was used to observe the morphology of red blood cells and the characteristics of secreting exosomes. Western Blot was used to detect the expression of phenotypes CD63 and CD81 of exosomes, and the expression of mitochondrial riboprotein MRPS35 of exosomes was also detected to explore the mechanism of decreased function of red blood cells with the extension of preservation time. Results: Changes in the rheological properties and oxygen-carrying functions of erythrocytes during the preservation process are all appearances, underlying which lies the damage mechanism at the molecular level of erythrocytes. Conclusion:Erythrocytes secrete exosomes, which resulted in abnormal expression of related proteins in mitochondria to increase ROS, mitochondrial apoptosis, and finally changes or damage to erythrocytes. Funding Statement: This work is supported by Key Disciplines Group Construction Project of Pudong Health Bureau of Shanghai (No.PWZxq2017-10), and National Natural Science Foundation of China (No. 81671919, 81870147). Declaration of Interests: All of the authors have no conflict of interest in this research. Ethics Approval Statement: Ethical approval was given by Gongli hospital, The Second Military Medical University.