Abstract
ABSTRACTThe cytotoxicity of a resin-based material can be evaluated on isolated human lymphocyte. Since resin-based dental materials have been used with increasing frequency in anterior and posterior teeth restorations, the uncured resin monomers are leached out from the restorations and diffuses into the dentine and ultimately hampers the odontoblastic layers of pulp as well as gingiva. It is also reaches into the saliva and circulatory blood. The study evaluates and compares the relative cytotoxicity of resin-based dental materials at different time interval, i.e. 24, 48, and 72 hours on human lymphocyte by Trypan blue exclusion method. All resins were found to be cytotoxic to human lymphocyte. Resin samples cytotoxicity was the highest in first 24 hours followed by 48 and 72 hours.
Highlights
Resins composites have been used as posterior restorative material with increasing frequency because of demand for both esthetic restorations and worries about adverse effects of mercury from amalgam (Sweeney et al 2002).[1]
Human lymphocytes were used for cytotoxicity testing in the study because they are sensitive cells that can be isolated as pure population from blood and cultured in normal culture medium
Among some 30 chemicals, the monomer 2-hydroxyethyl methacrylate (HEMA) and the co-monomer triethylene glycol dimethacrylate (TEGDMA) were detected (Santerre et al, 2001; Michelsen et al, 2003).[8]. Both HEMA and TEGDMA may diffuse through dentin in sufficient concentrations to cause cellular damage (Bouillaguet et al, 1996; Hume and Gerzina, 1996).[8]
Summary
Resins composites have been used as posterior restorative material with increasing frequency because of demand for both esthetic restorations and worries about adverse effects of mercury from amalgam (Sweeney et al 2002).[1] Chronic pulpitis were reported from the use of dental composites in cavities that have not been properly protected. Investigators such as Stanley et al (1978)[2] and Suarez et al (1978)[3] have recommended that composites be classified as toxic. Fujisawa et al (1999)[3] proposed that chemical irritation resulted from the hemolytic potency of BIS-GMA and other acrylates and vinyl monomers caused by the highly hydrophobic nature of the composites
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