Abstract
Anticipation of root canal bacteria contamination is the foundation of endodontic treatment. The most predominant type is Enterococcus Faecalis strain, along with which several Gram negative enteric rods or Gram positive facultative may also be present. The aim of the present study was to compare the bacterial reduction from the infected root canals taking samples of pre-chemomechanical and post-chemomechanical preparation by ProTaper Universal, WaveOne and NeoNiTi instrumentation systems. Seventy-five systematically healthy patients was selected with permanent mandibular premolar teeth with single root and single canal. They are divided in three: Group I (ProTaper Universal), Group II (WaveOne) and Group III (NeoNiTi), in which each individual pre and post instrumentation sample of microorganism was taken and then analyzed using Paired ‘t’ test, One Way ANOVA with Post HOC comparison using Tukey test for microbiological evaluation. When pre-chemomechanical samples were compared by applying One-Way ANOVA test, the difference in pre-chemomechanical values in all three groups was statistically insignificant (p>0.05). Statistical analysis by applying paired ‘t’ test, One-Way ANOVA and p value showed that there was highly statistically significant change from pre-chemomechanical values to post-chemomechanical values in each study group (p<0.001). There was highly statistically significant difference in CFU count percentage reduction between the pre-instrumentation and post-instrumentation values in all the groups (p<0.001). Group I (ProTaper Universal) showed the highest percentage reduction followed by Group III (NeoNiTi) and Group II (WaveOne). Statistical analysis by paired ‘t’ test and p value showed that the reduction in number of colony forming units from S1 (pre-instrumentation) to S2 (post-instrumentation) was statistically highly significant (p<0.001). It may be concluded that the most effective instrumentation technique in eliminating microorganisms from the root canal was ProTaper Universal system in comparison to WaveOne and NeoNiTi.
Highlights
Micro-organisms play a fundamental role in the aetiology of pulp and periapical diseases
Amongst the diverse bacteria species found in endodontic infections, Enterococcus Faecalis stands out for its ability to grow in the presence or absence of oxygen, penetrate deep into dentinal tubules and survive adverse environmental conditions such as extreme alkaline pH, high temperatures and scarce nutrition and association with persistent apical periodontitis, which makes it focus of research. [1]Reducing the bacterial count in infected root canals is accomplished by a combination of mechanical
When pre-chemomechanical samples were compared by applying One-Way ANOVA and p value, the difference in pre-chemomechanical values in all three groups was statistically insignificant (p>0.05) which means the prechemomechanical counts in all groups were in similar range
Summary
Micro-organisms play a fundamental role in the aetiology of pulp and periapical diseases. [4] During the past decades, many systems have come out with single file systems [20] i.e OneShape (Micro Mega, Bescanson, France), WaveOne (DentsplyMaillefer, Ballaigues, Switzerland), and new NeoNiTi (Neolix, Creative Dental Instruments, Chảtres-la-Forẻt, France), which claims that a single file can be used for the entire preparation of root canals and it allows easier, faster and better root canal shaping. These instruments are designed with various tapers, greater than the 0.02 taper of traditional instruments, may results in excessive removal of dentin and high stress generation within the root canals and weakening of the root
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