TL1A/DR3 interaction is associated with pathogenesis of inflammatory bowel disease in adults and children

Abstract

Event Abstract Back to Event TL1A/DR3 interaction is associated with pathogenesis of inflammatory bowel disease in adults and children Tomasz J. Slebioda1*, Piotr M. Wierzbicki1, Marcin Stanislawowski1, Krzysztof Celinski2, Piotr Landowski3, Barbara Kaminska3, Agnieszka Bojarska-Junak4, Jacek Rolinski4, Krystian Adrych5 and Zbigniew Kmiec6 1 Medical University of Gdansk, Department of Histology, Poland 2 Medical University of Lublin, Department of Gastroenterology with Endoscopic Unit, Poland 3 Medical Univesrity of Gdansk, Department of Pediatrics, Pediatric Gastroenterology, Hepatology and Nutrition, Poland 4 Medical University of Lublin, Department of Clinical Immunology, Poland 5 Medical University of Gdansk, Department of Gastroenterology and Hepatology, Poland 6 University of Warmia and Mazury, Department of Human Histology and Embryology, Poland TL1A is a proinflammatory cytokine and the ligand for death receptor 3 (DR3). These proteins are strongly up-regulated on activated cells of the immune system. TL1A/DR3 interaction costimulates T-cells and induces production of several proinflammatory cytokines (including IL-4, IL-13, IL-17A, IFN-γ) by these cells. TL1A and DR3 are linked to the development of Crohn’s disease (CD) and ulcerative colitis (UC), although their exact pathological role remains unknown. In this study, we investigated the mRNA level of TL1A, IL-4, IL-13, IL-17A and IFN-γ in colon mucosal biopsies of paediatric and adult inflammatory bowel disease (IBD) patients. We found that expression of TL1A was significantly elevated in inflamed but not in non-inflamed colonic tissue of both CD and UC patients as compared to healthy individuals. Interestingly, we detected up-regulation of DR3 only in inflamed colonic tissue of paediatric but not adult patients. Up-regulation of TL1A mRNA was accompanied by elevated expression of proinflammatory cytokines and positively correlated with the expression level of IL-17A mRNA in inflamed and non-inflamed tissue of both paediatric and adult UC patients. Furthermore, TL1A and DR3 protein expression was localized by immunohistochemical technique not only to mucosa-infiltrating mononuclear cells but also to enterocytes. Our study also provides evidence for the first time that TL1A mRNA expression is significantly higher while DR3 mRNA expression is significantly lower in healthy adults compared to healthy children. These findings show that TL1A contributes to the development of IBD via induction of IL-17A expression and this observation may have therapeutic implications. Keywords: TL1A, inflammatory bowel disease, DR3, TNFSF15, Inflammation, IL17A Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Slebioda TJ, Wierzbicki PM, Stanislawowski M, Celinski K, Landowski P, Kaminska B, Bojarska-Junak A, Rolinski J, Adrych K and Kmiec Z (2013). TL1A/DR3 interaction is associated with pathogenesis of inflammatory bowel disease in adults and children. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00153 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 11 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Tomasz J Slebioda, Medical University of Gdansk, Department of Histology, Gdansk, Poland, t.slebioda@gumed.edu.pl Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Tomasz J Slebioda Piotr M Wierzbicki Marcin Stanislawowski Krzysztof Celinski Piotr Landowski Barbara Kaminska Agnieszka Bojarska-Junak Jacek Rolinski Krystian Adrych Zbigniew Kmiec Google Tomasz J Slebioda Piotr M Wierzbicki Marcin Stanislawowski Krzysztof Celinski Piotr Landowski Barbara Kaminska Agnieszka Bojarska-Junak Jacek Rolinski Krystian Adrych Zbigniew Kmiec Google Scholar Tomasz J Slebioda Piotr M Wierzbicki Marcin Stanislawowski Krzysztof Celinski Piotr Landowski Barbara Kaminska Agnieszka Bojarska-Junak Jacek Rolinski Krystian Adrych Zbigniew Kmiec PubMed Tomasz J Slebioda Piotr M Wierzbicki Marcin Stanislawowski Krzysztof Celinski Piotr Landowski Barbara Kaminska Agnieszka Bojarska-Junak Jacek Rolinski Krystian Adrych Zbigniew Kmiec Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. 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