Abstract
Highly cross-linked (highly porous) polyacrylamide gels as described in the literature were found to be poorly characterized support media. Diallyltartardiamide (DATD) cross-linked gels contain up to 80–90% unpolymerized DATD, which reacts with proteins and produces gluey and highly stretchable matrices. The conversion into polymer could not be affected by time or higher temperatures, since DATD is an inhibitor of polymerization, but only by adding very high amounts of persulphate. Increased levels of riboflavin actually decreased the polymerization efficiency. Highly cross-linked N,N′-methylenebisacrylamide (BIS) gels, at 40–50% C, are too hydrophobic and produce a collapsed matrix which keeps exuding water. A happy compromise are 30% C BIS gels, which are stable and allow unhindered migration of globular proteins up to 5·10 5 daltons. BIS and DATD gels can be stabilized by covalent binding to a glass surface precoated with Silane A-174. The order of reactivity for monomers appears to be: acrylamide > BIS ⪢ DATD. Polymerization conditions are described which allow better than 96% conversion of monomers into the polymer.
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