Abstract
Lymphocytes of 50 patients were treated with ficin and typed for the HL-A antigens with a panel of allogeneic typing sera. The resulting phenotypes were identical to those obtained when the same cells were typed in our standard cytotoxicity test, although in three specimens the antigen 4b and in one the antigen HL-A7 could not be defined as clearly as in the standard test. In comparison with the latter test, a 2 to 5% incidence of false reactions was noted with the ficin test. The sensitivity of the ficin test as determined by titration of sera of four sensitized kidney transplant recipients was comparable to that of the standard cytotoxicity test. By using ficinized lymphocytes for tissue typing by the cytotoxicity method, the durations of the incubation times of the cells with the antisera and with heterologous rabbit complement could be markedly reduced. As a result, it is possible to tissue type recipients and donors for kidney transplants in half the time necessary to type with the standard cytotoxicity method.
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