TISSUE ENGINEERING MATRIX BASED ON POLYURETHANE: IN VITRO RESEARCH

Abstract

HighlightsThe article focuses on a new polyurethane-based material that has been developed and tested in vitro. This highly porous material with satisfactory physical and mechanical, hemocompatibility and matrix properties, obtained by using an electrospinning method, is suitable for the fabrication of cardiovascular products. AbstractAim. To manufacture a polyurethane-based tissue engineered matrix and study its physical and mechanical characteristics, hemocompatibility and matrix properties in comparison with decellularized xenopericardium and sheep carotid artery.Methods. Matrices based on polyurethane were produced by electrospinning. The surface structure was studied by scanning electron microscopy, the physical and mechanical characteristics were studied using a Zwick/Roell Universal testing machine, hemocompatibility was studied according to ISO 10993-4-2020, and the matrix properties of the material were studied in a cell experiment with Ea.hy 926.Results. The structure of the 12% polyurethane matrix was represented by a fibrous network with interpenetrating pores. The physical and mechanical characteristics of polyurethane matrices corresponded to the parameters of the carotid artery of sheep more than xenopericardium. Polyurethane had optimal hemocompatibility: hemolysis of erythrocytes did not exceed 0.52%, platelet aggregation corresponded to the aggregation of platelet-rich plasma – 80%. Platelet adhesion to the surface of the polyurethane matrix is statistically significantly lower than adhesion to the xenopericardium (p = 0.0041). Cell adhesion, viability and metabolic activity of Ea.hy 926 cultured on the surface of polyurethane matrices were higher relative to xenopericardium: cell density was 236.3 [198.5; 264.6] cells/mm2 (p = 0.458), viability 19.0 [16.0; 25.0] % (p = 0.0145).Conclusion. Physical and mechanical characteristics, hemocompatibility and matrix properties of polyurethane confirmed its suitability for potential use for the needs of cardiovascular surgery.