Abstract
The objective of this work was to establish tissue culture parameters for gene transfer in sweet orange cultivars. Epicotyl explants with different ages were cultured with 6-benzylaminopurine (BAP), kanamycin and hygromycin. Shoots were cultured with alpha-naphthaleneacetic acid (NAA) alone or in combination with indole-3-butyric acid (IBA). The requirement of BAP for shoot development was genotype-specific. Epicotyl explants from 35-day-old seedlings produced significantly more shoots per explant in 'Pêra'. Kanamycin inhibited shoot regeneration for the most cultivars. The percentage of shoots that produced roots in 'Pêra' was significantly higher in medium with NAA and IBA than with NAA alone.
Highlights
Resumo – O objetivo deste trabalho foi estabelecer parâmetros de cultura de tecidos para transferência gênica em cultivares de laranjeira-doce
A prerequisite for successful gene transfer to plants is the establishment of a suitable system of in vitro regeneration and recovering of whole plants from transformed cells
The supplementation of the growth regulator 6-benzylaminopurine (BAP) in the shoot regeneration medium is considered an absolute requirement, BAP concentration for optimal shoot regeneration varies and is considered to be genotype-specific (Bordon et al, 2000). Another parameter that influences the morphogenic response of plant material cultured in vitro is the age of the tissue or organ which is used as explant source (George, 1993)
Summary
Amanda Ferreira da Silva Mendes(1), Luciana Cardoso Cidade(1), Gabrielle Novais Manzoli(1), Wagner Campos Otoni(2), Walter dos Santos Soares Filho(3) and Marcio Gilberto Cardoso Costa(1). The supplementation of the growth regulator 6-benzylaminopurine (BAP) in the shoot regeneration medium is considered an absolute requirement, BAP concentration for optimal shoot regeneration varies and is considered to be genotype-specific (Bordon et al, 2000) Another parameter that influences the morphogenic response of plant material cultured in vitro is the age of the tissue or organ which is used as explant source (George, 1993). Elongated shoots (0.5 cm) of 'Pêra' and 'Pineapple' were, individualized and transferred to rooting medium (Costa et al, 2002), supplemented with either 1 mg L-1 NAA (alpha-naphthaleneacetic acid) or 0.5 mg L-1 NAA and 0.5 mg L-1 IBA (indole-3-butyric acid).
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