Abstract
The adsorption and transport processes of a hydrophobic molecular ion Malachite Green (MW 329.4) at E.coli bacterial outer membrane and cytoplasmic membrane, respectively, have been characterized by using a nonlinear optical technique- Second Harmonic Generation. Adsorption isotherms of the MG ion to both membranes of the gram-negative bacteria E.coli cell have been measured for the determination of the maximum adsorption densities and adsorption equilibrium constants. In some of the experiments, the classical permeablizer EDTA was used to eliminate the bacterial outer membrane and enable independent determination for the cytoplasmic membrane. A pH-modified Langmuir adsorption model has been applied to analyze the isotherms obtained under physiological conditions. The effect of solution ionic strength on adsorption has been examined. The nonlinear optical signal also directly allows the observation, with real time resolution, of the transport of the molecular ions through the two membranes and the determination of the respective transport rates.
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