Abstract
Time-resolved stimulated-emission fluorescence microscopy is a novel technique for obtaining super-diffraction limited spatial resolution and sub-nanosecond time resolution using a multi-photon process. This technique is inspired by traditional asynchronous stimulated-emission pump-probe spectroscopy. Fluorescence sample is first excited by a pump laser pulse, tuned to the molecular absorption band of the molecule. Within the chromophore lifetime, a second probe pulse, tuned to the emission band, stimulates fluorescence emission.The spatial resolution enhancement originates from the bilinear dependence of the stimulated emission efficiency on both the pump and probe beam intensities. At the objective focal point, the stimulated emission point spread function is the product of the point spread functions of the pump and probe beams. This situation is mathematically equivalent to both the confocal and the two-photon methods. 3-D depth discrimination and superior spatial resolution is expected.
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