Abstract
As the largest light-harvesting complex in cyanobacteria, phycobilisomes (PBSs) show high efficiency and a high rate of energy transfer, owing to an elegant antenna-like assembly. To understand the structural influence on the dynamic process of the energy transfer in PBSs, two cyanobacterium species Thermosynechococcus vulcanus NIES 2134 (T. 2134) and Synechocystis sp. PCC 6803 (S. 6803) with different rod–core-linked assemblies were chosen for this study. The dynamic process of the energy transfer in both PBSs was investigated through time-resolved fluorescence spectroscopy (TRFS) with a time resolution of sub-picosecond. Via the fluorescence decay curves deconvolution, the pathways and related rates of the excitation energy transfer (EET) were determined. Three time components, i.e., 10, 80, and 1250 ps, were identified in the EET in the PBSs of T. 2134 and three, i.e., 9, 115, and 1680 ps, in the EET in the PBSs of S. 6803. In addition, a comparison of the dynamic process of the energy transfer between the two cyanobacteria revealed how the PBS assembly affects the energy transfer in PBSs. The findings will provide insight into future time-resolved crystallography.
Highlights
Cyanobacteria are the most ecologically important phototrophic bacteria on earth
We investigated the dynamic process of EET among PBPs in the PBSs from two cyanobacteria species by using femtosecond FRTS and deconvolution method, giving the energy transfer pathways and the energy transfer time constants
The results indicate that differences in the rod–core assemblies can significantly affect the rod–core energy transfer dynamics in PBSs
Summary
Cyanobacteria are the most ecologically important phototrophic bacteria on earth. They constitute a phylogenetically coherent group of morphologically and evolutionarily ancient diverse bacteria [1]. Cyanobacteria are characterized by their capability to photosynthesize They synthesize PBSs as light-harvesting antennas composed of proteins that binding with various pigments. The geometry of a PBS molecule in cyanobacteria is an elegant antenna-like assembly, which is made up of phycobiliproteins (PBPs). A PBS molecule has a centrally located core composed of two to five allophycocyanin (APC), and in most cases surrounded by six to Crystals 2021, 11, 1233. The geometry of a PBS molecule in cyanobacteria is an elegant antenna-like asse2mobf l1y0, which is made up of phycobiliproteins (PBPs). A PBS molecule has a centrally located core composed of two to five allophycocyanin (APC), and in most cases surrounded by six to eight phycocyanin (PC) rods [7,8]. Inwoarvdeelerntgotehxspwloerreethdeecsotrnuvcotulurateldbafsoirs eosfttihmeaetinnegrgtyhetraensefregry mtreacnhsafenriscmominpoanceynatns.oIbnacotredreiar PtoBeSxapnloternenthae, tshterudcitfuferarelnbcaessisboeftwtheeeenntehregyenterargnysfterramnsefcehrkainiestmicsinofatchyeatnwoobcayctaenroiabaPcBteSraiantienndnuac,etdhbeyditfhferreondc-ecsorbeetawsseeemnbtlhyewenereergaynatrlyanzesdfeirnkdineetatiicl.s of the two cyanobacteria induced by the rod-core assembly were analyzed in detail
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
