Time Resolved Fluorescence Anisotropy on Supported Lipid Bilayers

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The diffusion and spatial organization of cell membrane receptor-ligand interactions, such as those involved in the formation of the T-cell immunological synapse, are important physical mechanisms of cell signaling. Although the binding kinetics within these structures have been established at the level of individual microclusters, the time-resolved interactions of single receptor-ligand pairs are generally not accessible by current optical methods. A fluorescence anisotropy method is proposed that has the potential to measure receptor-ligand kinetics without the need for intrinsic labeling of membrane receptors.