Time Kill Kinetic, Antibiofilm Activity and Effect on Biomolecules Release of Drypetes Gosswelleri and Echinops Giganteus Essential Oils on Bacteria

Abstract

Finding alternative strategies to confront bacterial resistance is an urgent need. Biofilm-forming bacteria have become a serious problem in medicine. Bacteria can use biofilm as a mechanism of resistance against antibacterial drugs. The aim of this study was to study times kill kinetic of Drypetes gosswelleri and Echinops giganteus essential oils, the antibiofilm formation activity and their effect on cell release compounds. Times kill kinetic was study by quantification of cellular growth over time in Petri dish after her inoculation by the cells treated with antibacteria at MBC and incubation at different times during 24h. The antibiofilm activity carried out by microdilution using 96 wells microplate. The production of biofilms by cells treated was observed and quantified after coloration by Crystal Violet dye and spectrophotometric dosage at 630nm. The effect on cell release compounds are study by determination of absorbent material in extracellular medium at 260nm after exposure at the antibacterial during 24h. The results obtained showed that, these EOs have an ability to kill bacterial cells over time, Drypetes gossweileri EOs like Ciprofloxacin, caused the death of all the cells in the inoculum treated before 14h of exposure of Staphylococcus aureus strain. Against Salmonella enteritidis strain, Drypetes gossweileri and Echnops giganteus EOs kill all the cells after 24h like the two antibiotics. One effect of the action these EOs on the strains elucidated in this study was the leakage of intracellular absorbent materials (DNA and RNA) this materialized a diminution of membrane permeability and cell wall integrity.