Abstract
Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) have gained great attention as a new green alternative to petrochemical-derived polymers. Due to their outstanding material properties they can be used in a wide range of applications. Pseudomonas putida KT2440 is a metabolically versatile producer of mcl-polyhydroxyalkanoates. Although the metabolism of polyhydroxyalkanoate synthesis by this bacterium has been extensively studied, the comparative proteome analysis from three growth stages of Pseudomonas putida KT2440 cultured with oleic acid during mcl-PHA synthesis has not yet been reported. Therefore; the aim of the study was to compare the proteome of Pseudomonas putida KT2440 at different time points of its cultivation using the 2D difference gel electrophoresis (2D-DIGE) technique. The analyses showed that low levels of a nitrogen source were beneficial for mcl-PHA synthesis. Proteomic analysis revealed that the proteins associated with carbon metabolism were affected by nitrogen starvation and mcl-PHA synthesis. Furthermore, the induction of proteins involved in nitrogen metabolism, ribosome synthesis, and transport was observed, which may be the cellular response to stress related to nitrogen deficiency and mcl-PHA content in bacterial cells. To sum up; this study enabled the investigators to acquire a better knowledge of the molecular mechanisms underlying the induction of polyhydroxyalkanoate synthesis and accumulation in Pseudomonas putida KT2440 that could lead to improved strategies for PHAs in industrial production.
Highlights
Synthetic plastics are considered one of the most problematic issues, and this has led to a growing interest in developing alternative materials that are environmental friendly
To evaluate the cellular responses during polyhydroxyalkanoates synthesis, Pseudomonas putida KT2440 was grown in nitrogen-limited mineral salt medium containing oleic acid as a carbon source
The analysis showed that amino acids, biosynthesis, The proteins that were differentially expressed during mcl-PHA synthesis under nitrogen and metabolism, and transport and binding were the top detected functional categories in all analyzed depletion were categorized into functional classes (Figure 5)
Summary
Synthetic plastics are considered one of the most problematic issues, and this has led to a growing interest in developing alternative materials that are environmental friendly. The various types of biopolymers polyhydroxyalkanoates (PHAs) are especially attractive due to their outstanding material properties, including biodegradability and bio-renewability, thermal plasticity, and biocompatibility. They have great potential as a new functional material in biomedicine, agricultural, and industrial applications [1,2]. PHAs are natural polyesters synthesized by a variety of bacteria as carbon and energy reserves, ensuring their survival during nutritional stress [3]. Culture stress is becoming an efficient strategy for the biosynthesis of polyhydroxyalkanoates by bacteria. It has been previously reported that nutrient limitation stimulates rapid PHA synthesis [4]
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