Abstract
This chapter discusses the mechanism of NaCl transport in the cortical thick ascending limb of Henle's loop. It describes that NaCl transport in this nephron segment produces a lumen positive electrical transepithelial potential difference (PD). This PD is eliminated by substituting large anions for chloride, by ouabain added to bath and by diuretics like furosemide added to the luminal perfusate. An improved method to record transepithelial electrical resistance (R T ) in conjunction with PD measurements at both ends of the isolated perfused cTAL segment has made it possible to investigate passive parameters, that is, the cation selectivity of the paracellular shunt pathway, and aspects of the active transport mechanism. Furosemide and ouabain experiments with high time resolution are performed to obtain some insight into the sidedness and mechanism of the inhibitor action. The chapter presents a model that is proposed for NaCl transport in cTAL in which a basolateral ATP dependent Na + -K + transport is the primary event. The lumen positive PD drives a large fraction of the Na + through the preferably sodium permeable paracellular shunt.
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