Abstract
In order to determine the intracellular pH (pH i ), a double-barreled liquid ion-exchanger (LIX) pH-microelectrode is constructed with nigericin and the tip is made less than 1 μm in outside diameter. This chapter illustrates the construction of the electrode. The electromotive force (EMF) of the LIX pH microelectrode shows a linear response to pH of test solutions within the range of pH 5–9. Various kinds of buffer solutions, such as citrate, phosphate, tris, and borate, are adequate for pH calibrations for this electrode. A couple of coaxial fiber-built-in capillaries are mounted in parallel on a micropipette-puller of horizontal type, rotated 180° under heated platinum ribbon, and pulled to a tip of less than 1 μm outside diameter. The chapter examines the effect of changes in external CO 2 on the pH on a single cell of perfused proximal tubule. It also describes the method of the perfused kidney preparation.
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