Abstract
BackgroundThe study aimed to investigate the expression changes of genes in colorectal cancer (CRC) and screen the potential molecular targets.MethodsThe GSE37178 of mRNA expression profile including the CRC samples extracted by surgical resection and the paired normal samples was downloaded from Gene Expression Omnibus database. The genes whose expressions were changed at four different time points were screened and clustered using Mfuzz package. Then DAVID was used to perform the functional and pathway enrichment analysis for genes in different clusters. The protein–protein interaction (PPI) networks were constructed for genes in the clusters according to the STRING database. Furthermore, the related-transcription factors (TFs) and microRNAs (miRNAs) were obtained based on the resources in databases and then were combined with the PPI networks in each cluster to construct the integrated network containing genes, TFs and miRNAs.ResultsAs a result, 314 genes were clustered into four groups. Genes in cluster 1 and cluster 2 showed a decreasing trend, while genes in cluster 3 and cluster 4 presented an increasing trend. Then 18 TFs (e.g., TCF4, MEF2C and FOS) and 18 miRNAs (e.g., miR-382, miR-217, miR-1184, miR-326 and miR-330-5p) were identified and three integrated networks for cluster 1, 3, and 4 were constructed.ConclusionsThe results implied that expression of PITX2, VSNL1, TCF4, MEF2C and FOS are time-related and associated with CRC development, accompanied by several miRNAs including miR-382, miR-217, miR-21, miR-1184, miR-326 and miR-330-5p. All of them might be used as potential diagnostic or therapeutic target molecules for CRC.
Highlights
The study aimed to investigate the expression changes of genes in colorectal cancer (CRC) and screen the potential molecular targets
The results indicated that three miRNAs were predicted to target genes in cluster 1: hsa-miR-382 could target CLDN1, DLX3 and IRX3; hsa-miR-217 could modeulate DMRT2, MSX2, SMAD9 and VSNL1; hsa-miR-21 could regulate PITX2 and VSNL1
In order to further investigate the pathogenesis and explore the molecular therapeutic methods for CRC, we re-analyzed the mRNA expression of CRC tissues extracted after surgical resection at four time points and screened a set of time-related genes, such as PITX2, VSNL1, TCF4, MEF2C and FOS (FBJ murine osteosarcoma viral oncogene homolog)
Summary
The study aimed to investigate the expression changes of genes in colorectal cancer (CRC) and screen the potential molecular targets. Colorectal cancer (CRC) is the third most common cancer worldwide with over 1,000,000 new cases every year [1]. In China, CRC is ranked as the fourth leading cause of cancer death with a pronounced increasing incidence during recent years [2]. The main risk factors for CRC include dietary and lifestyle factors, such as diet, obesity, physical activity, smoking and alcohol abuse [3,4,5]. Accumulating evidence indicate that multiple molecules are involved in CRC [6, 7]. For. Chen et al Cancer Cell Int (2016) 16:22. PTGS2 and ASPN) [12].
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