Time-course adaption strategy of Tetraselmis-based consortia in response to 17α-ethinylestradiol

Abstract

Estuarine ecosystem constitutes a microenvironment where the abundant green microalga Tetraselmis sp. co-exists with 17α-ethinylestradiol (EE2) pollution. However, the adaption mechanisms of this microalga-based consortia under EE2 shock are rarely recognized. Using extracellular polymeric substance (EPS) characterization, flow cytometry and transcriptomic, this study reveals the time-course response of Tetraselmis-based consortia under EE2 stress. Compared to the insignificant effect of 0.5 mg/L, a high dose of 2.5 mg/L EE2 induces persistent production of reactive oxygen species (ROS) and transiently physiological damages (membrane, chloroplast, organelle morphogenesis, and DNA replication), resulting in cell cycle alteration and division inhibition. These damages could be recovered through active DNA repair and persistently detoxifying processes of enhanced metabolism and ROS quenching. The enhanced EPS production is observed and in line with the significant up-regulation of most key enzymes involved in precursor synthesis and polysaccharides assembling. However, the up-regulation of glycoside hydrolases and most glycosyltransferases, down-regulation of flippases and changed expression of ABC family members indicate the changed EPS composition and synthesis strategy. The resulting increased colloidal polysaccharide is further consumed by associated bacteria whereas protein remains in the co-cultures. These results provide deeper insights into the adverse effects of chemical compounds to microalgae-bacteria and their coadaptation ability.