Abstract
AbstractOocytes at different stages of growth were isolated from the guppy and incubated with and without increasing doses (0.01 to 1000 ng/ml) of a tilapia prolactin (tPRL188). Measurement of the estradiol‐17β secreted into the medium showed that estrogen synthesis from endogenous precursors was stimulated by tPRL at all stages of oocyte growth. In all cases, the maximal stimulating dose was 1 ng/ml. At this dose, the magnitude of the stimulation in estradiol‐17β synthesis was greatest (77%) in oocytes at the beginning of vitellogenesis. These results strongly suggest a role of prolactin in ovarian steroidogenesis of the guppy, and it is possible that this action of prolactin constitutes an additional control mechanism regulating estrogen synthesis in the fish.
Published Version
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