Abstract
Among the brain tumors, Glioma is the most malignant disease that causes death and the prognosis of patients remains poor. Tight junctions (TJs) are important in formation of endothelial cells of the blood-brain barrier (BBB) and acts as a restrictive paracellular diffusion barrier. Claudins are imp components of TJs, and expression of these proteins were altered selectively in cerebral microvessels of human GBM and other pathological conditions, resulting in brain edema. The aim of this study was to determine the pattern of expression of tight junction associated protein claudin 3 in low grade and high grade glioma cell lines C6, U373, U118, T98 and U87MG using Real Time PCR and western blot methods. Interestingly quantitative Real Time PCR and western blot results have shown down regulation of claudin 3 in high grade glioma to low grade glioma. Our results concluded that decreased expression of claudin 3 was correlated with disease progression.
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call