Abstract
A primary goal in modern surface modification technology of dental implants is to achieve biocompatible surfaces with rapid but controlled healing which also allow health and longevity of implants. In order to realize all, understanding of osseointegration phenomena is crucial. Although Ti-SLA, Ti-SLActive and TiZr-SLActive surfaces have been successfully used in clinical implantology and were shown to notably reduce the primary healing time, available in vitro studies are sparse and do not concern or explore the mechanism(s) involved in human osteoblast behavior on these surfaces. Ti-SLA, Ti-SLActive, TiZr-SLActive, Ti cp, Ticer and Cercon surfaces were used. Osteoblast proliferation, cell cluster formation, morphological changes, induction of autophagy, nitric oxide (NO), reactive oxygen species/reactive nitrogen species (ROS/RNS) formation, osteocalcin (OC), bone sialoprotein (BSP) and collagen type I (Col-1) affected by various surfaces were analyzed. These surfaces induced formation of mature osteoblasts caused by elevated oxidative stress (ROS) followed by overexpression of osteoblast maturation key molecule (NO), with different intensity however. These mature osteoblasts induced upregulation of OC, BSP and Col-1, activating PI3/Akt signalling pathway resulting in autophagy, known as an important process in differentiation of osteoblast cells. Additional distinctive subpopulation identified on Ticer, Ti-SLA (after 5 days), Ti-SLActive and TiZr-SLActive surfaces (after 2 days) were forming cell clusters, essential for bone noduli formation and mineralisation. The results suggest that Ti- and TiZr-SLActive possess advanced properties in comparison with Ticer and Ti-SLA manifested as accelerated osteoblast differentiation. These effects could explain already known fast osseointegration of these surfaces in vivo.
Highlights
The development of novel dental implant materials as well as elucidation of cellular and acellular healing mechanisms between implant surface and surrounding solid and soft tissues have been in progress for over six decades, i.e., from Brånemark’s discovery demonstrating that titanium could become permanently incorporated into bone [1,2]
Several different topographies employed clinically as dental implants were used in this study: Ti cp, Cercon, Ticer, Ti-SLA, Ti-SLActive and clinically
Smooth topography material served as the controls, because they basedinvestigated on zirconiain(Cercon) was used for [21,48]
Summary
The development of novel dental implant materials as well as elucidation of cellular and acellular healing mechanisms between implant surface and surrounding solid and soft tissues have been in progress for over six decades, i.e., from Brånemark’s discovery demonstrating that titanium could become permanently incorporated into bone [1,2]. Since it has been shown that physicochemical properties (chemical composition, surface roughness, micro/nanotopography, wettability, surface charges, etc.) have the most important influence on cell adhesion, proliferation, differentiation and bone matrix deposition [4,5], major improvements in material surface development have been achieved in order to reduce the critical confronts of the implant dentistry. Many years of research resulted in several improved materials with advanced mechanical properties, as well as enhanced biocompatibility and osseointegration [9,10]. Successful functional and excellent esthetic solutions were awaited, and short and predictable healing time enabling fast loading of implants
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