Abstract
BackgroundTo explore the pathological changes of three-dimension structure of ventricular myocardial fibers after anterior myocardial infarction in dog heart.MethodsFourteen acute anterior myocardial infarction models were made from healthy dogs (mean weight 17.6 ± 2.5 kg). Six out of 14 dogs with old myocardial infarction were sacrificed, and their hearts were harvested after they survived the acute anterior myocardial infarction for 3 months. Each heart was dissected into ventricular myocardial band (VMB), morphological characters in infarction region were observed, and infarct size percents in descending segment and ascending segment were calculated.ResultsSix dog hearts were successfully dissected into VMB. Uncorresponding damages in myocardial fibers of descending segment and ascending segment were found in apical circle in anterior wall infarction. Infarct size percent in the ascending segment was significantly larger than that in the descending segment (23.36 ± 3.15 (SD) vs 30.69 ± 2.40%, P = 0.0033); the long axis of infarction area was perpendicular to the orientation of myocardial fibers in ascending segment; however, the long axis of the infarction area was parallel with the orientation of myocardial fibers in descending segment.ConclusionsWe found that damages were different in both morphology and size in ascending segment and descending segment in heart with myocardial infarction. This may provide an important insight for us to understand the mechanism of heart failure following coronary artery diseases.
Highlights
To explore the pathological changes of three-dimension structure of ventricular myocardial fibers after anterior myocardial infarction in dog heart
Echocardiography Echocardiography confirmed that old myocardial infarction was successfully established in the 6 dogs, in which dyskinesia was found in the left anterior wall and apex
Morphologic characteristics of VMB Six hearts were successfully dissected into ventricular myocardial band (VMB) (Figure 1), which was composed of basal and apical loops as Torrent-Guasp described [5]
Summary
Experimental preparation: Establishing the model of acute myocardial infarction in dog [4] Fourteen dogs received humane care in compliance with the 1996 NRC Guide for the Care and Use of Laboratory Animals. They were offered by Animal Experimental Center of PLA General Hospital. To measure the infarct size more exactly, we calculated infarct size percents of descending segment and ascending segment respectively with a new method which was designed based on Torrent’s double helical VMB theory rather than a traditional method. The infarct size percents of descending segment and ascending segment were calculated respectively as follows: ISPDS = 100% × IADS / ADS.
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