Abstract
Three new spectrum filtration protocols have been developed and adapted to overcome some difficulties in dealing with highly overlapping triple drug mixtures by proposing new smart mathematical techniques that facilitate the resolution of the ternary mixture and the recovery of a filtrated zero-order spectrum (D0 spectrum) of each component without any overlapping from the accompanying components. The three established spectrophotometric protocols were conducted on the combination of ciprofloxacin hydrochloride and ornidazole as a green alternative to the usual chromatographic technique: the first protocol is ratio difference-isosbestic points coupled with ratio difference-areas under the curve (RD-ISO/RD-AUC); the second protocol is ratio difference-isosbestic points coupled with dual-wavelength equation (RD-ISO/DWE); and the third protocol is signal retrieval by zero-crossing point (SRZ). All three developed protocols have the power to recover a filtrated zero-order spectrum of each ornidazole and ciprofloxacin hydrochloride without any involvement from the ciprofloxacin-induced degradation substance through processing their spectral data either in the zero-order spectrum, ratio spectrum, or derivative spectrum. The correctness of the spectral filtration process for each protocol was checked by involving the spectral print recognition index to ensure the drug's purity and freeness from impurities or degradation products. The validation process was performed as per the directions of ICH, which confirmed the effectiveness of the elaborated protocols and their usability as daily analysis methods with a linearity range of (3.5–15 μg/ml) for ciprofloxacin in (RD-ISO/RD-AUC) and (RD-AUC/DWE) protocols and (1.5–15 μg/ml) in (SRZ) protocol; and a linearity range of (3–20 μg/ml) for ornidazole in (RD-ISO/RD-AUC) and (SRZ) protocols and (3–15 μg/ml) in (RD-ISO/DWE) protocol. A statistical comparison and greenness evaluation utilizing NEMI, AGREE, GAPI, and CALIFICAMET-HEXAGON tools were made with the reference approach, confirming no statistical variations and a better greenness profile for the newly established protocols.
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