Abstract
Abstract Scaffolds of collagen and collagen with hyaluronic acid were fabricated using 3D printing and crosslinked by dehydrothermal treatment. Rheological measurements of both these dispersions revealed high values of viscosity and a shear thinning behaviour. Fourier transform spectrometry indicated an increased amide bone formation when hydrothermal treatment was applied, with scanning electron microscopy revealing different pore size distributions in the scaffolds with micro- and nano-fibres. The cross-linking treatment in both scaffolds improved resistance to collagenase and elastic mechanical properties, whereas those with hyaluronic acid showed higher resistence solubilization than scaffolds without treatment in a short time at physiological conditions. The swelling ratio of all crosslinked scaffolds decreased, but this treatment prevented disintegration of collagen with hyaluronic acid scaffolds in aqueous medium. Additionally, the cytotoxicity for Vero and NIH3T3 cells showed that the collagen scaffold extracts were not cytotoxic, whereas extracts from collagen plus hyaluronic acid crosslinked scaffolds were cytotoxic only for Vero cells. In vitro evaluation using BJ cells from human skin demonstrated that all scaffolds had the ability to support cell attachment and proliferation. These results revealed that the properties of the 3D printed scaffolds based on collagen and hyaluronic acid could be modified by dehydrothermal treatment, with the materials obtained provide the support required in tissue engineering.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.