Abstract
Vibrio cholerae resistance to third-generation cephalosporins is rarely reported. We detected a strain that was negative for extended-spectrum β-lactamase and positive for the AmpC disk test, modified Hodge test, and EDTA disk synergy test and harbored the blaDHA-1 and blaNDM-1 genes. The antimicrobial drug susceptibility profile of V. cholerae should be monitored.
Highlights
Vibrio cholerae resistance to third-generation cephalosporins is rarely reported
Before the child received antimicrobial therapy, a fecal specimen was submitted to the Department of Microbiology at JIPMER
Antimicrobial drug susceptibility testing was conducted by Kirby–Bauer method in accordance with the Clinical and Laboratory Standards Institute [4] against ampicillin (10 μg), ceftriaxone (30 μg), ciprofloxacin (5 μg), furoxone (300 μg), cotrimoxazole (25 μg), and tetracycline (30 μg)
Summary
Vibrio cholerae resistance to third-generation cephalosporins is rarely reported. We detected a strain that was negative for extended-spectrum β-lactamase and positive for the AmpC disk test, modified Hodge test, and EDTA disk synergy test and harbored the blaDHA-1 and blaNDM-1 genes. Reported resistance to most commonly used antimicrobial drugs, i.e., tetracycline and ciprofloxacin [1], has limited options for therapy. Such drug-resistant V. cholerae strains threaten public health [2]. Antimicrobial drug susceptibility testing was conducted by Kirby–Bauer method in accordance with the Clinical and Laboratory Standards Institute [4] against ampicillin (10 μg), ceftriaxone (30 μg), ciprofloxacin (5 μg), furoxone (300 μg), cotrimoxazole (25 μg), and tetracycline (30 μg). We performed the combination disk test using cefotaxime and ceftazidime, alone and in combination with clavulanic acid, to detect extended-spectrum β-lactamase (ESBL) in accordance with Clinical and Laboratory Standards Institute guidelines [4]. We used the BLASTN program (www.ncbi.nlm.nih.gov/ BLAST) for database searching
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