Abstract
Aim: The purpose of this study was to evaluate the relation between chronic hepatitis B (CHB) and the thiol/disulfide balance, used as a marker of oxidative stress. Materials and Methods: This study was conducted between May 2016 and July 2016 at the Erzurum Regional Training and Research Hospital Infectious Diseases Polyclinic. 63 subjects with CHB and 60 healthy volunteers without any known diseases were included in the study. In term of measuring dynamic thiol/disulfide homeostasis, we used the novel automated assay method developed by Erel and Neselioglu. Results: Native thiol (SH), total thiol (total SH) and disulfide (SS) levels were determined; measures such as SS/SH, SS/total SH, and SH/total SH were calculated. It is determined that CHB group’s SH levels (P=0.041), total SH levels (P=0.043) were lower than the control group’s. There is negative correlation between Anti-HBc total IgG and total SH, SH, SH/total SH, there is positive correlation between Anti-HBc total IgG and SS/SH, SS/total SH ratio. There is negative correlation between BMX index and total SH, SH, SH/total SH and positive correlation between SS/total SH, SS/SH. Positive correlation is determined between total protein, albumin and total SH, SH. Conclusions: In our study thiol levels as an antioxidant were found to be low in CHB patients. Thiol levels were again emphasized as a new marker in hepatitis B. Thiol levels in CHB are thought to shed light to slowing of disease course and improving new treatment efforts with more wide studies.
Highlights
Hepatitis B infection is common in our country and in the world and is the leading infection among viral infections that become chronic [1]
hepatitis B virus (HBV) infection is clinically observed in the form of acute infection, chronic infection or primary hepatocellular carcinoma (PHC)
While chronic hepatitis is seen in 5-10% of patients, cirrhosis and hepatic failure may develop in 10% of those with chronic hepatitis. 80% of cases with PHC are associated with chronic HBV infection [11]
Summary
Hepatitis B infection is common in our country and in the world and is the leading infection among viral infections that become chronic [1]. Free oxygen radicals are influential in reactions caused by various drugs/toxins such as lead poisoning (toxicity), carbon tetrachloride-induced hepatic damage, and in glomerulonephritis, hepatitis B, ischemia, vitamin (C and E) deficiency, and in the pathogenesis of many diseases such as cancer, emphysema, hyper oxidation, bronchopulmonary dysplasia, atherosclerosis, pancreatitis and rheumatoid arthritis [3]. Antioxidants trying to prevent the damaging effects of free radicals, especially thiol groups, cannot preserve the plasma and tissue levels during these affections [5]. Thiols are a class of organic compounds that contain a sulfhydryl group (-SH) which is composed of a hydrogen and a sulfur atom attached to a carbon atom [6] They are compose the basic part of the nonenzymatic antioxidant system in the circulation, and are the first molecules responsible for neutralization of oxidant molecules [7]. While the serum thiol/disulfide levels were previously assessed indirectly, direct measurement possibility has been available with fully-automated calorimetric technique which was found by Erel and Neselioglu in 2014 [9]
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