Thin-layer and overpressured-layer chromatography for evaluation of the hydrophobicity ofs-triazine derivatives

Abstract

The retention factor in pure water, log k w , is proposed as a measure of the hydrophobicity of s -triazine derivatives. In these studies two reversed-phase techniques, thin-layer chromatography (TLC) and overpressured-layer chromatography (OPLC), were used. The effects of the chromatographic system, i.e. mobile and stationary phase properties, on log k w were analyzed to select the best system for determination of the hydrophobicity of the compounds. In TLC the chemically bonded phases RP-18 and RP-CN were used as stationary phases and acetone, acetonitrile, tetrahydrofuran, and dioxane as organic modifiers of the mobile phases. In OPLC RP-8 and RP-18 stationary phases and water-acetonitrile mobile phases were used. The values of log k w , determined by linear extrapolation and by the new method based on Ościk’s equation, were compared with log P values calculated by different theoretical methods. In TLC the best linear correlations between log P and log k w were observed for water-dioxane as mobile phas...