Abstract
A new method of specimen illumination for wide-field fluorescence microscopy has been presented. This method allows to excite the fluorescence in a thin near-surface layer of the studied object. As a result, the captured images have greater contrast and signal-to-background ratio in comparison with the epifluorescence ones. The long-range surface waves in one-dimensional photonic crystal have been used to localize the electromagnetic field exciting the fluorescence. An experimental setup has been created to excite the surface waves and obtain images of the objects from the near-surface layer. For an illustration of the possibilities of our method, we conducted several experiments with specimens that are typical for fluorescence microscopy, such as bacteria and eukaryotic cells.
Highlights
One-dimensional photonic crystal (1D PC) is a multilayer meta-structure with periodically changing refractive indices of the layers
Konopsky et al [1], it is possible to create the special conditions for existence of one or several modes of longrange surface optical range electromagnetic waves on the surface of 1D PC
The main idea of our work [2] is to use a small penetration depth of the long-range surface waves to improve the contrast and signal-to-background ratio of the images that could be captured with the help of a wide-field fluorescence microscope
Summary
One-dimensional photonic crystal (1D PC) is a multilayer meta-structure with periodically changing refractive indices of the layers. Konopsky et al [1], it is possible to create the special conditions for existence of one or several modes of longrange surface optical range electromagnetic waves on the surface of 1D PC. The main idea of our work [2] is to use a small penetration depth of the long-range surface waves to improve the contrast and signal-to-background ratio of the images that could be captured with the help of a wide-field fluorescence microscope. One of the most frequently used methods of such kind image improvement is the so-called Total Internal Reflection Fluorescence Microscope (TIRFM).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
