Abstract
Several excellent solvents for the thin-layer chromatography (TLC) of vitamin B12 and the related compounds were found out using Aluminium oxide G, Kieselgel G and Kieselguhr G as inorganic adsorbents, and P-cellulose as well as DEAF-cellulose as organic ones. Since there are many vitamin B12 factors of similar Rf values, it is still difficult to separate each factor in the mixture completely and identify it. It is advisable to prepurify or fractionate prior to the TLC of vitamin B12.
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