Abstract
Ceramides with mono-, di-, and trihydroxy long-chain bases, and normal (saturated and unsaturated), branched-chain, and 2-hydroxy fatty acids have been analyzed by thin-layer chromatography. In most cases the compounds were also run as acetates. Borate, arsenite, and silver ions were used as complexing agents, and the effects of number, position, and stereochemistry of hydroxy groups, and of unsaturation, were studied. The results are discussed in view of analysis of natural ceramide species.
Highlights
Ceramides with mono, di, and trihydroxy long-chain bases, and normal, branched-chain, and 2-hydroxy fatty acids have been analyzed by thin-layer chromatography
Ceramide species differing only in unsaturation do not separate on gasliquid chromatography (GLC) [18, 19], and homologous series differing in the number of hydroxy groups may overlap [19, 21, 22]
The preparation of the long-chain base derivatives listed in Table 1 will be published eslewhere.' Acetylation was done with acetic anhydride and pyridine [26]
Summary
Ceramides with mono-, di-, and trihydroxy long-chain bases, and normal (saturated and unsaturated), branched-chain, and 2-hydroxy fatty acids have been analyzed by thin-layer chromatography. CERAMIDES (N-acyl derivatives of long-chain bases) are the lipophilic parts of all natural sphingolipids. Due to the existence in nature of about 60 bases (for a review, see Ref. 11) and at least as many sphingolipid fatty acids [12], a great number of ceramide species may exist.
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