Abstract
Monoglycosylceramides of bovine kidney have been separated on columns of borate-impregnated silica gel G into six fractions homogeneous on thin-layer plates. The lipophilic components were identified by mass spectrometry of trimethylsilyl ethers of intact lipids, and the carbohydrate identified by gas chromatography after degradation. Dihydroxy and trihydroxy long-chain bases, normal and 2-hydroxy fatty acids, and glucose and galactose were the components of eight possible combinations. Two of these made up 90 % of the total weight, namely glucosylceramide with trihydroxy base and hydroxy fatty acid, and galactosylceramide with dihydroxy base and hydroxy fatty acid.
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
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