Abstract
Abstract Thiabendazole and benomyl are extracted from fruit peels with ethyl acetate and the extracts are filtered. The filtrates are extracted with 0.1N HC1, adjusted to pH 6 with 0.1N NaOH, and re-extracted with ethyl acetate. The concentrated extracts are spotted on plates coated with silica gel GF-254. The fungicides are detected on the plates under shortwave ultraviolet light, after development with chloroform-acetone (8+2), as pink-blue spots on a light fluorescent background. The recovery of thiabendazole from fortified samples is satisfactory, but the recovery of benomyl is not quantitative. For greater specificity, an enzymatic inhibition technique is also described which may be used on the exposed silica gel GF-254 plate or on a silica gel G plate.
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