Abstract
Background: Tuberculosis (TB) remains one of the major causes of death from a single infectious agent worldwide. The early detection of new cases of pulmonary tuberculosis is an important goal in tuberculosis control program.Objective: 1n this study, thin layer agar (TLA) culture was compared with Lowenstein-Jensen (LJ) culture for rapid detection of pulmonary tuberculosis. Methods: It was a cross sectional study conducted in National Tuberculosis Reference Laboratory (NTRL) of National Institute of Disease of Chest and Hospital (NIDCH), Dhaka, from July 2010 to June 2011. A total of 100 sputum smear positive for acid fast bacilli (AFB) by Z-N staining, pulmonary tuberculosis patients were included in this study. Samples were processed by modified Petroff method and then cultured on thin layer 7H11(TL7H11) plates and L-J tubes. TL7H11 plates were observed microscopically for rnicrocolony growth once a week for 6 weeks, and L-J tubes were observed once a week for 8 weeks. Results: The recovery rates of mycobacteria on only TLA, only LJ and on both media were 90%, 97% and 88% respectively. Overall positivity was 99% in both L-J and TLA media. Mean time for detection of mycobacteria on TLA was 9.04±1.66 days compared to 21.78±6.19 days on L-J media. The rate of contamination was higher (6%) in L-J media than in TLA media (4%). Conclusion: The TL7H11 media can be used as an alternative to the Lowenstein-Jensen medium for early isolation of mycobacteria in resource constrained settings.
Highlights
Despite the discovery of the tubercle bacillus more than a hundred years &Eo, and,all the advances in our knowledge of the disease made since tuberculosis still remains one of the major health problems facing mankind, in developing countries
A cross sectional study conducted in National Tuberculosis Reference Lfuoratory National Tuberculosis Reference Laboratory (NTRL)) of National Institute of Disease of Chest and Hospital (NIDCH), Dhaka, from July 2010 to June 2011
Suspected pulmonary tuberculosis patients referred to the NTRL by physicians were enrolled for sputum examination
Summary
Despite the discovery of the tubercle bacillus more than a hundred years &Eo, and,all the advances in our knowledge of the disease made since tuberculosis still remains one of the major health problems facing mankind, in developing countries. It is estimated that currently there are about 10 million new cases of tuberculosis every year with 3 million deaths occurring world-widel. More people die of tuberculosis than from arry other infectious disease. Tuberculosis (TB) remains one of the major causes of death from a single infectious agent worldwide. Objective: In this study, thin layer agar (TLA) culture was compaxed with Lowenstein-Jensen (LJ) culture for rapid detection of pulmonary tuberculosis. Methods: It was a cross sectional study conducted in National Tuberculosis Reference Laboratory (NTRL) of National Institute of Disease of Chest and Hospital (NIDCH), Dhaka, from July 2010 to June 201 1. Samples were processed by modified Petroff method and cultured on thin layer 7H11(TL7H11) plates and L-J tubes .TLTHll plates were observed microscopically for microcolony growth once a week
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