Abstract
Understanding of the cellular role of nucleic acid triple helices and utilization of triple-helix forming oligonucleotides in biotechnology, diagnostics, and therapeutics depend on development of an understanding of triple-helix formation as a function of the nucleic acid components and solution conditions. This article reviews developments in nucleic acid triple-helix thermodynamics with emphasis on the construction and interpretation of state diagrams as a means of characterizing the complex behavior of triple-helix forming oligonucleotides.1
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