Abstract
The interaction between anionic form of copper (II) N,N',N",N'"-tetrasulfonated phthalocyanine Cu (tspc) and to calf thymus deoxyribonucleic acid (ct-DNA) is investigated by measuring UV-vis absorption and fluorescence spectroscopy in phosphate buffer. The binding constant and stoichiometry were determined by analysis of optical absorption spectra of phthalocyanine at various ct-DNA concentrations using SQUAD software. The static mode of fluorescence quenching of phthalocyanine by calf thymus deoxyribonucleic acid indicates the formation of a ground-state complex. The formation of ground-state complex is a spontaneous molecular interaction procedure in which outside groove binding through the formation of an axial bond between the base pairs of nucleotide and Cu in the central core of phthalocyanine.
Highlights
The interaction of small molecules with DNA continues to be an extremely important area of research, in the sense of both fundamental and practical point of view, as the molecular recognition of DNA is of fundamental importance to life
The special properties of porphyrins and metalloporphyrins, e.g., high absorbance, relatively high quantum yields of fluorescence and triplet state or paramagnetism of some metal complexes lead the use of porphyrin in medicine, as active compounds in radiological [7,31] and magnetic resonance imaging [3,20] of cancer detection
In this paper we have investigated the interaction of an anionic phthalocyanine with calf thymus DNA
Summary
The interaction of small molecules with DNA continues to be an extremely important area of research, in the sense of both fundamental and practical point of view, as the molecular recognition of DNA is of fundamental importance to life. Dezhampanah et al / The study on the binding of interaction anionic phthalocyanine with calf thymus DNA major groove or the shallow minor groove of the DNA helix and (c) outside binding with self-stacking involving porphyrins are stacked along the DNA helix. Cationic porphyrins are considered as double functional compounds that strongly bind to DNA and photodynamically modify the target site of a DNA molecule by a mechanism similar to that of anti-cancer antibiotics such as bleomycin and daunomycin based on the DNA cleavage [2,14,22]. Phthalocyanines differ from porphyrins by having nitrogen atoms link the individual pyrrol units They have been extensively studied as DNA binders. H. Dezhampanah et al / The study on the binding of interaction anionic phthalocyanine with calf thymus DNA 359 mode of binding and binding constant using SQUAD software. Running the interaction experiments at various temperatures, let us to estimate all of the thermodynamic parameters of interactions and obtain useful information regard binding mechanism
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