Abstract
This study investigated the influence of thermally modified tourmaline (Tur) on hydrogen production during the dark fermentation of corn stover. Single-factor experimental results revealed influencing factors of particle size, mass, and temperature. Optimization of the experimental process was achieved using the Box-Behnken design, reaching optimum at conditions of 407 °C, 910-mesh, and 6.2 g. The principle analysis experiment showed that the Tur-enhanced group (Tur_En) amplified cumulative hydrogen production by elevating hydrogen production during the sugar-production stage. The Tur_En group's cumulative hydrogen production was measured at 396.2 ± 40.3 (mL/g VS), marking a 34.2% increase compared to the control group. Analysis of microbial diversity indicated that Firmicutes and Bacteroidota emerged as dominant colonies in both stages. Tur facilitated hydrogen production by stimulating the activity of Firmicutes. This study suggests a highly effective Tur-enhanced technology for hydrogen production from corn stover and elucidates the principles underpinning this method from two stages.
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