Bio-electrolytic conversion of acidogenic effluents to biohydrogen: An integration strategy for higher substrate conversion and product recovery

Abstract

Feasibility of integrating Microbial electrolysis cell (MEC) process with dark-fermentation process for additional hydrogen recovery as well as substrate degradation was demonstrated in the present study. MEC was employed in order to utilize the residual organic fraction present in the acidogenic effluents of dark fermentation process as substrate for hydrogen production with input of small electric current. MEC was operated at volatile fatty acids (VFA) concentration of 3000mg/l under different poised potentials (0.2, 0.5, 0.6, 0.8 and 1.0V) using anaerobic consortia as biocatalyst. Maximum hydrogen production rate (HPR), cumulative hydrogen production (CHP) (0.53mmol/h and 3.6mmol), dehydrogenase activity (1.65μg/ml) and VFA utilization (49.8%) was recorded at 0.6V. Bio-electrochemical behavior of mixed consortia was evaluated using cyclic voltammetry and by Tafel slope analysis. Microbial diversity analysis using denaturing gradient gel electrophoresis confirmed the presence of γ-proteobacteria (50%), Bacilli (25%) and Clostridia (25%).