Abstract
Aim: In order to decipher the mechanisms underlying the known protective effects of the thermal water from Uriage-les-Bains (TWFULB) on the skin barrier function, we studied its antioxidant properties as well as its effect on the expression of the tight-junctional protein claudin-6, a candidate tumor suppressor factor. Study Design/Methods: In a first step, TBARS and SOD activity assays were performed in an in vitro model of human dermal fibroblasts treated by hypoxanthine/xanthine oxidase (HO/XO) mixture, in order to evaluate the own antioxidant effect of the thermal water. In a second step, human keratinocytes irradiated or not by UVB were used to evaluate the protective role of TWFULB on nuclear DNA damage using a comet assay. In a third step, an ex vivo model of human skin explants irradiated or not by UVA and UVB was used to evaluate the effect of TWFULB on the intracellular catalase activity and on the cutaneous claudin-6 expression. Results: TWFULB showed significant protective effects against oxidative stress induced by HO/XO: the cell viability was improved and the lipid peroxidation was reduced. The tested thermal water also showed significant SOD-like activity and protective effect on the UVB-stressed DNA. Considering the ex vivo models of skin explants, TWFULB was able to counterbalance the “negative” effect of UVB on the intracellular catalase activity and on the cutaneous claudin-6 expression.Conclusion: This multiparametric approach shows the antioxidant activity of TWFULB and emphasizes its role in the DNA protection of the cutaneous tissue in front of the UV irradiations, and finally suggests that some effects could involve the candidate suppressor functions of claudin-6.
Highlights
Numerous cosmetic products contain thermal spring waters coming from different origins and showing different physiological effects including in relation to their ionic compositions
We demonstrated the beneficial effect of the thermal water from Uriage-les-Bains (TWFULB) on the skin barrier recovery after injury
TWFULB was able in the same time to reduce the lipid peroxidation induced by the hypoxanthine/xanthine oxidase (HX/XO) stress
Summary
Numerous cosmetic products contain thermal spring waters coming from different origins and showing different physiological effects including in relation to their ionic compositions. Sulphurous thermal waters for example, have shown antioxidant properties [1]-[3], DNA protective effects [1] and inhibitory activities on neutrophil elastase [2]. “La Roche Posay” thermal water for its part is rich in selenium, and displays antioxidant, anti-inflammatory, immunodulating and anti-irritant potential [6]. Another example coming from Korea clarified the action of the spa spring water from “Yong-gung oncheon” on cytokine expression in human keratinocyte HaCaT cells and on differentiation of CD4+ T Cells [7]. In addition to these considerations, the evaluation of the potential activity of a thermal water in preventing the nuclear DNA damages induced by UV irradiations in cutaneous tissue, and on the subsequent consequences is a crucial, and yet relatively unexplored issue
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
