Abstract
The present study aims toevaluate the prevalence and antimicrobial sensitivity ofStaphylococcus aureus associated with bovine mastitis toselected antibiotics and plant extracts. Inthe current study, 140milk samples were collected from cows and buffaloes. Among the 140samples, 93samples were positive for sub-clinical mastitis based onthe California Mastitis Test (CMT). Out ofthe total positive samples, 45 were confirmed for S.aureus onaMannitol salt agar media. The antimicrobial susceptibility test revealed that 44.82% ofthe isolates were resistant tocefoxitin (oxacillin) confirming methicillin-resistant S.aureus (MRSA) with ahigher percentage (51.61%) inthe buffalo than inthe cow samples. Furthermore, the PCR assay confirmed the presence ofthemecA gene inall the MRSA isolates. Among the seven tested antibiotics, sulfamethoxazole + trimethoprim showed high efficacy (71.1%) against methicillin-susceptible S.aureus isolates (MSSA). Oxytetracycline and sulfamethoxazole+ trimethoprim showed 20% efficacy against MRSA followed byenrofloxacin (10%). Onthe other hand, the tested samples from Pistacia chinensis revealed that the ethyl acetate extract ofbark showed amaximum zone ofinhibition of21.3mm against MSSA and MRSA isolates at3000μg/disc. Moreover, the methanol extract ofCotoneaster microphyllus formed a12.3mm and 9.1mm zone ofinhibition against the MSSA and MRSA isolates, respectively.
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