Abstract
Plasma Activated Media (PAM) has shown an increasing anticancer effect in the past few [1-3]. In in vitro, decrease in cell proliferation [2.4], DNA damage [2;5] and apoptosis [6] were observed. The main advantage of PAM is its possibility to be prepared in advance and it can be used several weeks after activation by plasma jet when it is stored at the right temperature [2;7]. The aim of this work is to investigate the inhibitory effect of PAM and hydrogen peroxide H2O2 on Head and Neck cancerous cells (FaDu). The response of FaDu cells in monolayer cultures and Multi Cellular Tumor Spheroids (MCTS) after treatment with different PAMs was compared. A homemade helium plasma jet was used to produce PAM. Helium plasma jet was generated using dielectric barrier discharge configuration. Helium was injected with flowmeter and the gas flow is fixed at 3L/min. One treatment with PAM induces cell detachment from MTCs since the first day in a PAM exposure dependent manner. This is due to the presence of H2O2 in PAM. However, a rapid spheroids regrowth is observed attributed to a resistance of FaDu cells to H2O2. After multiple treatments of MCTS with PAM we obtained an inhibition of cell growth. MCTS is brought out with a comparison of PAM effect on 2D versus MCTS. Inversely, PAM induces cell death in the case of 2D cell culture. This result underlines the importance of working with MCTS instead of 2D cells.
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