Abstract
Low-temperature plasma (LTP) has been shown to have killing effects on cancer cells both in vitro and in vivo [1] – [5]. One of the approaches in treating mammalian cells with LTP is through plasma activated media (PAM). PAM is produced by exposing cell culture media to plasma. While the adverse effects of PAM treatment on cancerous cell lines have been recently studied, less is known about the effects of PAM on normal healthy cells. In this presentation, non-cancerous canine kidney MDCK (Madin-Darby Canine Kidney) epithelial cells are treated by PAM. Time-lapse microscopy is used to monitor cell proliferation and random migration after PAM exposure. It is found that moderate levels of PAM treatment inhibited cell proliferation, but longer exposure leads to substantial cell death. In addition, PAM treatment reduces random cell migration within epithelial islands. Immunofluorescence staining shows that while there are no major changes in the actin/adhesion apparatus, there is a significant change in the nuclear localization of proliferation marker Ki-67, consistent with the time-lapse results.
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