Therapeutic and Antioxidant Potential in the Shell Extract of Prunus amygdalus against Dermal Mycosis

Nasreen Thebo

doi:10.4172/2167-0412.1000108

Abstract

Mycosis is a common harmful problem for health throughout the world. Ringworm infection is one of the most dermatomycoses caused by species of pathogenic fungi such as Epidermatophyton, Trichophyton and Microsporum. The potential of biotechnology is to provide health protection through medicinal plants because it also facilitates the earlier detection of various ailments by new biotechnological techniques. Almond posses a variety of benefical properties affecting most notably cancer, high blood pressure, infectious disease and diabetes. In our study, we have used first time Almond hard shell extract for the evolution of antioxidant, antimicrobial potential and therapeutic efficacy against dermatomycosis pathogenic fungi. The extract was prepared by soxhlet method and TPC in the extract was determined by spectrophotometerically. The antioxidant activity of crude extract of almond shell was evaluated by using DPPH (2-2-diphenyl-1-picryhydrazyl) and radical scavenging system. The Minimum Inhibition Concentration (MIC) of extract was determined by well method against isolated pathogenic causative organisms for confirmation. The possibility of short term therapy was only 15 days. The total antioxidant activity varied from 93.32 to 94.40% and total phenolic content was found 3.422 mg/gm in almond shell extract. Hard shell extract of almond was found to be most effective as an antifungal and antibacterial agent against human pathogenic. Finally the results provide a therapeutic potential for microbial infections.

Highlights

The Almond, (Prunus amygdalus), apparently originated from lower mountain slopes of central south western, Mediterranean region and Asia from which about 28% of the world production is obtained [1]
Preparation of the almond hard shell extract: The hard shell of almond powdered with the help of warring blender. 15 gm of dried powder was filled in thimble and macerated with 100 ml of analytical grade solvent methanol
Total phenolic contents were determined by modified Folin Ciocalteu reagent was used for the determination of the total phenolic compounds and results are expressed as Gallic acid equivalents

Summary

Introduction

The Almond, (Prunus amygdalus), apparently originated from lower mountain slopes of central south western, Mediterranean region and Asia from which about 28% of the world production is obtained [1]. Almonds (Prunus amygdalus Miller D.A. Webb) are a rich source of phytochemicals and nutrients such as monounsaturated fatty acids, vitamin E, and polyunsaturated fatty acids [3]. Prunus species keeps biological properties such as antiinflammatory sedative, anti-hyperlipidemia, antitumor and antioxidant activities [2]. The natural antioxidants that scavenge reactive oxygen species are known to be of great value in preventing from oxidative stress and its related diseases [3]. Research proved that out of nine phenolic compounds in almonds exhibit strong antioxidant potential. Other workers reported that almond nuts are a source of, phytochemicals, proteins, unsaturated fats, minerals, micronutrients, alpha-tocopherol, and dietary fiber [7,8,9,10]

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Conclusion