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Abstract
The article presents the results of the research on methods of identification and quantitative determination of prion proteins in biological samples and multicomponent mixtures based on them. Analysis of nucleotide sequence of DNA encoding the PRNP gene of the prion protein, including phylogenetic and comparative analysis of nucleotide sequences of normal and pathogenic prion protein in cattle, was performed. Oligonucleotide primers for amplification of the PRNP gene of pathogenic prion protein were designed and synthesized. The high specificity of the developed test system was confirmed.
Highlights
Prions are a special class of purely protein agents, free of nucleic acids, causing severe diseases of central nervous system in human and a number of higher animals [1,2,3].Prion protein can exist in two forms: a noninfectious vitally important protein present in the organism of mammals, including human, and an infectious protein, which is a mutation of the normal prion protein causing prion diseases of animals and man.Prion diseases are a group of transmissive neurodegenerative diseases of animals and humans
In the Enactment of the Chief State Medical Officer of the Russian Federation no. 15 of 15.12.2000 «On the Measures for Prevention of Creutzfeldt–Jacobs Disease Spreading on the Territory of the Russian Federation», preventing measures aimed at prohibition of import of diseased meat and meat products were defined for the first time
We did not succeed to optimize the conditions for separation of cattle whole blood electrophoretic separations, we analyzed the blood plasma which was obtained by centrifugation at 3000 rpm for 5 min
Summary
Prions (proteinaceous infectious particles) are a special class of purely protein agents, free of nucleic acids, causing severe diseases of central nervous system in human and a number of higher animals [1,2,3]. Prophylaxis of prion diseases is based on prohibition of the infected meat products or other killing products on food market In this connection, in the Enactment of the Chief State Medical Officer of the Russian Federation no. 15 of 15.12.2000 «On the Measures for Prevention of Creutzfeldt–Jacobs Disease Spreading on the Territory of the Russian Federation», preventing measures aimed at prohibition of import of diseased meat and meat products were defined for the first time. Taking this into account, improvement and development of new methods for identification of prion proteins in biological material is of scientific and practical interest
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