Abstract
The incorporation of [14C]uridine into RNA by cultured rat epididymal tubules was tested after different times in culture. Incorporation after 3 days in culture was twice that found after 1 day in culture, and reached a maximum after 5 days. The presence of androgen in the culture medium for a 3-day period produced significant stimulation of [14C]uridine incorporation into RNA. 10 μM Dihydrotestosterone (DHT) or testosterone (T) caused 52 and 68% increases, respectively. 0.1 μM DHT enhanced incorporation by 79%. The effects of androgens became detectable only 10 hours after the addition of the hormone. Neither estradiol-17ß, progesterone nor corticosterone was able to mimic the effect of DHT or T, while 10 μM 5α-androstane-3α-17ß-diol produced a 40% stimulation ofuridine uptake. The effect of 10 μM DHT was blocked by the simultaneous presence of 0.1 mM cyproterone acetate.Fetal calf serum contains 0.35 ng T/ml. When the culture media were prepared with charcoal-treated serum in which T is undetectable by radioimmunoassay, the tissues responded with increased uridine incorporation to the addition of 5 nm DHT or T. Under these conditions, 1 μM DHT covalently linked to bovine serum albumin was without effect.
Published Version
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