Abstract
Three methods of staining myelin sheaths by the Weigert-Pal method in paraffin sections are compared. Hyomandibular nerves and spinal cord of the ratfish (Hydrolagus colliei) and tibial and peroneal nerves of the white rat were used. Fixation and mordanting comprised: 10% neutral formol, 48 hr.; 2.5% potassium bichromate at 37 °C., 12 days; gliabeze, 2 days. The tissue was washed for 24 hr., and stained for 24 hr. either in bulk, or while in paraffin sections, or on the slide after coating with celloidin. Kulschitzky's haematoxylin, freshly prepared and artificially ripened, was used for staining. Differentiation was carried out by Pal's method. Bulk staining gave most satisfactory results, with the other methods as second and third alternatives, respectively. Treating the sections with safranin and dehydrating in dioxan was found to give a satisfactory counterstain.
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