The visible to the near infrared narrow band acousto-optic tunable filter and the hyperspectral microscopic imaging on biomedicine study

Abstract

Based on the parallel tangents momentum-matching condition, a narrow band noncollinear acousto-optic tunable filter (AOTF) using a single TeO2 crystal is designed with the consideration of the birefringence and the rotatory property of the material. An effective setup is established to evaluate the performance of the designed AOTF. The experimental observed spectrum pattern of the diffracted light is nearly the same with the theoretical result. The measured tuning relationship between the diffracted central optical wavelength and acoustic frequency is in accordance with the theoretical prospect. The optical bandwidth of the diffracted light is as narrow as 1.88 nm when the central wavelength is 556.75 nm. The high spectral resolution is significant in practical applications of imaging AOTF. Additionally, the AOTF based hyperspectral microscopic imaging system is established. The stability and the image resolution of the designed narrow band AOTF are satisfying. Finally, the study of the biologic samples indicates the feasibility of our system on biomedicine.