High-Resolution Hyperspectral Microscopic Imaging With Single Acousto-Optic Tunable Filter Based on Double Filtering

Xiaofa Zhang,Zhenfei Sheng,Pengchong Wang,Chunguang Zhang,Yuhao Liu,Xiansheng Wang,Weijie Qiu,Haiping Tong,Yupuyun Wang,Hao Wang,Wenyao Liu,Xi Huang,Zhiwei Tan

doi:10.1109/access.2019.2963369

Abstract

Hyperspectral imaging is a technique that integrates multiple spectral bands and image information. Its applications range from improving the accuracy of cancer diagnosis to testing the quality of products. Here, we introduce a double-filtering technique that provides high-resolution diagnostic histological images within minutes. The hyperspectral microscopic imaging system is built based on an acousto-optic tunable filter (AOTF). The optimized system is analyzed from the perspective of spectrum and imaging. The spectral resolution can be improved by 37.08 % to 59.95% in the visible light range. The side lobe is obviously inhibited and the purity of spectrum is improved. Furthermore, the example of hyperspectral microscopic imaging is demonstrated with unstained gastric cancer tissue sections to assess the ability of the system in terms of its spectral performances and image quality. The microscopic imaging results of single filtering optical path system and single crystal double filtering optical path system are compared. In general, the optimized double filtering system achieves excellent performance in bandwidth compression and side lobe suppression, especially the first application of hyperspectral microscopy combined with microscope in the visible light range.

Highlights

Hyperspectral imaging (HSI) technology is a growing field integrating multiple spectral bands and spatial information
We present an optimized hyperspectral microscopic imaging system with single acousto-optic tunable filter (AOTF) based on double filtering for increased spectral resolution, decreased side lobe and improved image quality [9]
RESULTS we report on the performances of the single and double filtering with a TeO2 crystal, including the tuning relationship of the wavelength with radio frequency (RF), spectral bandwidth, spectral resolution and side lobe, using broadband light source

Summary

INTRODUCTION

Hyperspectral imaging (HSI) technology is a growing field integrating multiple spectral bands and spatial information. X. Zhang et al.: High-Resolution Hyperspectral Microscopic Imaging With Single AOTF Based on Double Filtering. Jullien introduced a polarized hyperspectral imaging with two cascaded liquid crystal cells [19] For the latter, You described the double filtering based on AOTF and confirmed the feasibility of the method with a HeNe laser and a diode laser [20]. On the basis of the previous work [27], we have successfully realized the micro-imaging in the broadband light range by combining the double filtering system with the inverted microscope for the first time. We present an optimized hyperspectral microscopic imaging system with single AOTF based on double filtering for increased spectral resolution, decreased side lobe and improved image quality [9]. The microscopic imaging results of single filtering optical path system and single crystal double filtering optical path system are compared.The proposed single crystal double filtering system has many advantages, such as low cost, high resolution, high quality image, and compact, integrated with microscope

EXPERMEBTAL SETUP

RESULTS

SPECTRAL ACQUISITION AND ANALYSIS

CONCLUSION